不同林龄杉木林下套种阔叶树对土壤磷组分的影响

套种是杉木人工林经营的重要措施,磷是南方森林生态系统中主要限制性养分元素之一,但套种模式对土壤磷素的影响尚不明确。以亚热带杉木人工林表层(0-10 cm)土壤为对象,研究套种林(杉阔套种幼林、杉阔套种成熟林)和杉木幼林土壤理化性质和土壤各形态磷含量差异,分析套种对杉木人工林土壤磷含量的影响。结果表明:(1)不同套种林显著改变土壤总磷、土壤总无机磷、土壤总有机磷、土壤微生物生物量磷(MBP)和土壤酸性磷酸酶活性(APA),大小顺序均为杉阔套种成熟林>杉阔套种幼林>杉木幼林。(2)土壤各磷组分中活性磷含量较低,其中NaHCO3-Po在活性组分中占主导;土壤NaOH-Po是中等活性磷的主要组分,杉阔套种成熟林尤为显著;闭蓄态磷(Residual-P)在总磷含量中最高。(3)与杉木幼林相比,杉阔套种成熟林显著增加了树脂提取态磷(Resin-Pi)、碳酸氢钠提取态有机和无机磷(NaHCO3-Pi、NaHCO3-Po)、氢氧化钠提取态有机和无机磷(NaOH-Pi、NaOH-Po)、氢氧化钠残留提取态有机磷(NaOHu.s-Po)、盐酸提取态磷(HCl-Pi)和闭蓄态磷(Residual-P)含量;土壤总无机磷、NaHCO3-Po、HCl-Pi、NaHCO3-Pi、NaOHu.s-Pi和Residual-P对杉阔套种幼林的响应不敏感。(4)除含水率外不同林龄下杉阔套种林土壤磷形态与土壤理化性质(土壤总碳氮、土壤可溶性有机氮、土壤微生物生物量磷、酸性磷酸酶)呈正相关性(P<0.05)。冗余分析表明,土壤磷组分的变化主要受MBP调控,且MBP与有机磷组分(NaOHu.s-Po、NaOH-Po)和HCI-Pi呈显著正相关。总之,套种林的土壤磷素有效性高于杉木幼林,土壤养分状况更佳。     

施肥对复垦土壤微生物代谢功能与苗期玉米生长的影响

利用Biolog-ECO技术、WinRHIZO扫描仪及其图像分析技术，研究了对照CK（施肥量为0）、施复合肥F（施肥量为600 kg·hm^-2）、施有机肥O（施肥量为7 500 kg·hm^-2）、有机无机肥配施OF（施肥量为复合肥300 kg·hm^-2+有机肥3 750 kg·hm^-2）处理下煤矸石复垦区土壤微生物群落代谢功能及苗期玉米根系形态等特征，结果表明：不同施肥处理下煤矸石复垦区土壤微生物群落功能多样性差异显著，OF处理下土壤微生物多样性Shannon-wiener指数与Simpson指数最高，分别为3.22和0.96， Eveness指数最低，为0.21，而CK处理与之相反，Shannon-wiener指数与Simpson指数最低，分别为3.07和0.95，Eveness指数最高，为0.23；影响该区域土壤微生物群落代谢功能多样性的关键碳源包括6种氨基酸（L-精氨酸、L-天冬酰胺酸、L-苯基丙氨酸、L-丝氨酸、L-苏氨酸、甘氨酰-L-谷氨酸)、4种酯（D-半乳糖酸γ内酯、丙酮酸甲酯、吐温40、吐温80）、1种胺（腐胺）、5种酸（D-半乳糖醛酸、2-羟苯甲酸、4-羟基苯甲酸、r-羟基丁酸、D-苹果酸）、3种糖（-甲基D-葡萄糖苷、葡萄糖-1-磷酸盐、a-环状糊精）、3种醇（I-赤藻糖醇、D,L-a-甘油、D-甘露醇）；OF处理下玉米根系形态参数如根长、根体积、根系分形维数及玉米生物性状如生物量、茎粗、株高等与CK处理差异最为显著，说明OF处理有利于煤矸石复垦土壤质量的恢复与提高，利于煤矸石复垦区玉米生长。     

冰淇淋混合料液的老化

阐述了冰淇淋混合料液老化过程的条件控制及混合料在老化过程中发生的主要变化。经过老化过程,混合料液的黏度显著增加,有助于凝冻过程中的搅打起泡,使冰淇淋产品的质构更加细腻,抗融性和贮藏稳定性更好。     

Effects of cover crop growth and decomposition on the distribution of aggregate size fractions and soil microbial carbon dynamics

Although the effects of cover crops (CC) on various soil parameters have been fully investigated, less is known about the impacts at different stages in CC cultivation. The objective of this study was to quantify the influence of CC cultivation stages and residue placement on aggregates and microbial carbon (C_mic). Additionally, the influence of residue location and crop species on CO₂ emissions and leached mineralized nitrogen (N_min) during the plant degradation period was also investigated. Within an incubation experiment, four CC species were sown in soil columns, with additional columns being kept plant‐free. After plant growth, the columns were frozen (as occurs in winter under field conditions) and then incubated with the plant material either incorporated or surface‐applied. With CC, concentrations of large and medium macroaggregates were twice that of the fallow, confirming positive effects of root growth. Freezing led to a decrease in these aggregate size classes. In the subsequent incubation, the large macroaggregates decreased far more in the samples with CC than in the fallow, leading to similar aggregate size distributions. No difference in C_mic concentration was found among the CC cultivation stages. CO₂ emissions were roughly equivalent to the carbon amounts added as plant residues. Comparison of columns with incorporated or surface‐applied residues indicated no consistent pattern of aggregate distribution, CO₂ emission or C_mic and N_min concentrations. Our results suggest that positive effects of CC cultivation are only short term and that a large amount of organic material in the soil could have a greater influence than CC cultivation.     

微生物菌剂肥对超级杂交水稻生长和产量的影响

以两优959为供试水稻,设计了0、150、300和450 g/667m2 4种不同施用量处理,考察微生物菌剂肥对超级杂交水稻生长发育及产量的影响。结果表明,以常规施肥+300 g/667m2微生物菌剂肥的处理效果最好,与对照相比,其单株平均分蘖数多了2.4个,有效穗多了11.05%,产量增加了10.3%。这说明微生物菌剂肥在超级杂交水稻生产上具有较高推广应用价值。     

Effects of phytosterol ester on aortic aging and expression of related genes in rats

AIM: To explore the protective effect of phytosterol ester (PSE) on aortic aging in rats. METHODS: The female SD rats (12 months old, n=42) were randomly divided into control group, model group and PSE group. During the experiment, the rats in control group, model group and PSE group were treated with basic feed, high-fat diet (HFD) and HFD with 2% PSE (W/W) for 6 months, respectively. The morphological changes of the aorta were observed by HE staining and Masson staining, and the absolute area of smooth muscle cells and collagen fiber in the vascular wall were measured by image analysis. The levels of advanced glycosylation end products (AGEs), malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) in the plasma were detected. The expression of silent information regulator 1 (SIRT1) and peroxisome proliferator-activated receptor γ (PPARγ) at mRNA and protein levels in the vascular tissue was determined by real time PCR and Western blot, respectively. RESULTS: PSE significantly lowered plasma TC and LDL-C, and increased plasma HDL-C level (P<0.05), but had no effect on plasma TG level. PSE significantly attenuated the thickening of intima and media of aging aortic, and decreased the migration of vascular smooth muscle cells (VSMC) and the amount of VSMC and collagen fiber in the aorta (P<0.05). PSE significantly reduced the contents of AGEs and MDA (P<0.05), but had no effect on the activity of SOD and CAT in the plasma. PSE also down-regulated the expression of PPARγ and up-regulated the expression of SIRT1 (P<0.05). CONCLUSION: PSE is able to attenuate the senescence process in the aorta by reducing the production of reactive oxygen species in plasma, and activating SIRT1, or inhibiting the expression of PPARγ in vascular tissues.     

艾纳香内生真菌抗细菌和炭疽菌的活性研究

为获得丰富的艾纳香内生真菌资源,筛选出抗性生防菌株,用于活性次生代谢产物的发现,通过水琼脂法对海南产艾纳香进行内生真菌分离,通过ITS序列分析,对其进行鉴定和分析。采用滤纸片法评价内生真菌发酵产物对细菌的抑制活性,采用平板对峙培养法评价艾纳香内生真菌对植物炭疽菌的拮抗作用。本研究共分离鉴定出191株内生真菌,分属于27属,45种,其中优势菌属为Diaporthe、Paraphoma、Ectophoma、Penicillium、Talaromyces、Hypoxylon、Phomopsis、Colletotrichum和Fusarium。抗细菌活性结果显示,Clonostachys、Fusarium和Diapothe属活性较强。抗炭疽病菌试验显示,Talaromyces、Ectophoma和Penicillium属活性较强。本研究初步探讨了海南产艾纳香内生真菌种类的多样性,并首次对其进行了抗病原菌活性筛查,获得的活性菌株可作为微生物源菌剂开发的重要材料。     

白三叶返园对苹果园土壤微生物群落的影响

为了解苹果(Malus pumila)园中覆盖和埋置白三叶(Trifolium repens)对土壤微生物群落的影响,采用尼龙袋法将新鲜的白三叶覆盖和埋置到苹果园中,分别在白三叶降解1、3、6个月时,利用Biolog微平板法检测土壤微生物群落的功能多样性。结果表明,覆盖和埋置白三叶均可使苹果园土壤微生物代谢活性显著提高(P0.05),微生物的多样性除6个月外显著增加、丰富度显著增加,均匀度显著降低(P0.05),群落结构发生变化,且覆草处理大于埋草,同时,白三叶降解时间的长短也影响土壤微生物群落。土壤微生物群落主成分分析结果显示,不同返园方式间主要对羧酸类碳源的利用有差异,而导致不同降解时间土壤微生物群落代谢差异的主要是糖类。说明在苹果园覆盖和埋置白三叶可改变土壤微生物群落,而覆盖白三叶对微生物群落结构的改善情况显著优于埋置白三叶。     

三峡库区不同植被覆盖对土壤氮的影响

不同植被类型影响着土壤养分的积累、分布与循环,而土壤氮素是植被生长的重要限制性元素。通过分析宜昌点军区3种植被类型(柏树地、橘树地、菜地)覆盖下土壤氮素的变化情况,研究了不同植被对土壤氮素各形态的影响。结果表明,土壤全氮、硝态氮和微生物氮都是柏树地显著大于菜地和橘树地,而菜地和橘树地之间无显著性的差异;土壤矿化氮和微生物氮/全氮的变化顺序是柏树地橘树地菜地。说明不同植被覆盖对土壤氮有显著的影响,柏树地更有利于土壤氮的积累,氮的有效性也最高,由此认为柏树长期生长有益于土壤氮的改善。     

Soil CO2 emission in sugarcane management systems

Sugarcane management systems affect soil attributes such as the carbon cycle. This fact has stimulated the sugar and alcohol industry to refine the sugarcane production systems by replacing the pre-harvest burning (PB) and manual harvest with mechanized harvesting followed by residue deposition. The aim of this study was to evaluate different management systems with respect to C cycling carbon dioxide and soil parameters (chemical, physical and biological) which were determined over the season. Three sugarcane cultivation systems were evaluated at the following periods: (a) PB, (b) 5-year green harvest and (c) 10-year green harvest. The results indicated that CO₂ emission was 36% greater in the 10-year sugarcane green harvest system than in the PB system. The bulk density and macroporosity were the factors that were most affected by the different sugarcane management systems and that significantly influenced soil CO₂ emissions. The principal component analysis showed that soil CO₂ emission was 18% influenced by base saturation (V%) and 14% by pH, especially in the PB area. Additionally, 19% was affected by carbon and macroporosity in the 5- and 10-year green harvest areas, respectively. From our results, it can be concluded that the most CO₂ emissions are in the areas of sugarcane green, this is due to the higher carbon concentration when compared with the area of burning sugarcane. The parameters that most influenced the CO₂ emissions were bulk density, porosity, macroporosity, pH and V%.